Journal: Frontiers in Molecular Biosciences
Article Title: Transcriptomic profiling of chlorogenic acid and taurine treatment in human skin cells provides insights into cellular senescence mechanisms
doi: 10.3389/fmolb.2026.1748185
Figure Lengend Snippet: Inferred transcriptomic architecture of antioxidant-responsive DEGs. (A) , Number of identified antioxidant-responsive DEGs in each human skin cell type. Bar heights indicate the number of DEGs identified under each treatment condition, with values labeled above each bar. Dark-colored segments represent the subset of AR-DEGs among the antioxidant-responsive DEGs. Bar colors correspond to different treatment conditions. (B) , Regulatory network of DEGs inferred using DoRothEA. Circles represent genes with directed edges from transcription factors to their predicted targets based on the DoRothEA regulon. Nodes are colored by log 2 FC from differential expression analysis in epidermal keratinocytes (above) and melanocytes (below). Gene symbols and log 2 FC values are labeled on each node. Genes with |log 2 FC| ≤ 0.585 are shown with lighter colors and gray text. Aging-related DEGs are marked with an asterisk next to the gene names. (C) , Antioxidative and anti-inflammatory pathways associated with DEGs. Each bar represents a pathway or GO term ( y -axis) identified in this study, with the corresponding −log 10 adjusted P -values from gprofiler2 ( x -axis). The vertical dashed line indicates the significance threshold of adjusted P -value = 0.05. (D) , A selected molecular model illustrates interaction between treatment of CGA and taurine and genes related with anti-aging mechanisms. Abbreviations: CGA, chlorogenic acid; CGA + Tau, combined treatment of chlorogenic acid and taurine; log 2 FC, Bayesian shrinkage estimator for log 2 fold change.
Article Snippet: Normal Human Epidermal Keratinocytes (Cat. no. PCS-200–010; ATCC, Manassas, VA, United States) were cultured in Keratinocyte Growth Medium (Lonza, KGMTM Gold, Cat. no. 00192060; BS, Switzerland) supplemented with 10% fetal bovine serum (Gibco, Waltham, MA, United States), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco).
Techniques: Labeling, Quantitative Proteomics
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